polynucleotide kinase การใช้

XRCC1 complexes with polynucleotide kinase ( PNK ) which processes DNA termini.
DLA probes are ligated at the 5'and 3'ends of the analyte by the joint action of T4 polynucleotide kinase.
Blunt-ended PCR product normally lacks a 5'- phosphate, therefore it needs to be phosphorylated by treatment with T4 polynucleotide kinase.
The second linker interacts with polynucleotide kinase phosphatase ( PNKP ) ( that processes DNA broken ends during base excision repair ), aprataxin ( active in single-strand DNA repair and non-homologous end joining ) and a third protein designated aprataxin-and PNKP-like factor.
:Kinases are enzymes that facilitate the addition of phosphate groups to other molecules; a'kinased'oligonucleotide is therefore one to which a phosphate has been added ( generally to the 5'end ) through the use of an enzyme ( most often T4 or T7 polynucleotide kinase ) . talk ) 15 : 49, 30 April 2012 ( UTC)